renovate kitchen cabinets cheap Scheme summarizing the different type of events observed in the first 20 min after inducing lysosomal damage. Graph shows the percentage of single G3BP1+ events, GAL-3+ events or combined GAL-3+G3BP1+ events observed by live-cell imaging. e, Spatial point pattern analysis applied to G3BP1+ and GAL-3+ events. In box plots, the centre line is the median, boxes delineate the interquartile range (IQR), and whiskers represent the data range within 1.5 times the IQR. n = 40 events examined over 3 independent experiments; P value by Monte Carlo simulation-based approach. f, G3BP1 polarized ‘plug pattern’ on GAL-3+ damaged vesicles and the corresponding 3D z-stack reconstruction. g, Quantification and schematic of plugging events shown in f. n = 40 events examined over 3 independent experiments. SG, stress granule. h, Image sequence (1 s frame rate, shown at 5s intervals) of iPSDMs expressing G3BP1–GFP (magenta) and GAL-3–RFP (green) after 2 min of LLOMe treatment. i, Mean fluorescence intensity (MFI) over time of G3BP1+ and GAL-3+ events as shown in h. Graphs show traces over time and standard error. n = 30 events examined over 3 independent experiments. AU, arbitrary units. j, Live-cell imaging sequence of iPSDMs expressing LAMP1–RFP and G3BP1–GFP. Arrowhead indicates membrane rupture site. The panel on the far right shows a 3D reconstruction. k, MFI of G3BP1–GFP over time for sequence shown in j. Bar plots indicate mean ± s.e.m. of at least three independent experiments. Scale bars: 10 μm (a, top), 2 μm (a, middle, bottom, f,h,j). Credit: Nature (2023). DOI: 10.1038/s41586-023-06726-w” width=”800″ height=”530″>
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